GC‐Ms, UHPLC–MS/MS ⁿ Phytoconstituents Characterization and Physio‐pharmacological Investigation of Silene colorata subsp . ampho

ABSTRACT

Natural remedies combined with bioinformatics accelerate the formulation of safer, targeted drugs. This study provides a comprehensive description of the phytochemical and pharmacological aspects of Silene colorata . Binding profiles of eight identified compounds were simulated against α‐amylase. GC‐MS analysis revealed 57 compounds, mainly fatty acids and esters (73.65%), while UHPLC–MS/MS identified 2′,4′‐dihydroxychalcone (20.72%), ( epi )gallocatechin‐ C ‐hexoside (12.53%), apigetrin (10.10%), and hexahydroxyheptanoic acid (8.57%) as major components. The hydro‐alcoholic extract showed a strong antiradical effect (IC ₅₀  = 7.57 ± 0.26 µg/mL by ABTS assay; A _0.50  = 48.93 ± 3.88 µg/mL by phenanthroline assay). Its sun protection factor (SPF) was also the highest (40.561 ± 0.87). The chloroform fraction showed notable activity against α‐amylase (IC ₅₀  = 22.86 ± 3.19 µg/mL) and butyrylcholinesterase (IC ₅₀  = 111.08 ± 4.72 µg/mL). The complex 3‐tetradecyn‐1‐ol and α‐amylase appeared more stable, but in an antagonistic manner (−4.8 kcal/mol). While this work reveals crucial findings, further in vivo studies are needed to confirm and expand on these results.