Fractionation of Ocimum sanctum (Tulsi) Extract and Evaluation of Its Antimicrobial, Antioxidant and Anti-Inflammatory Properties with Potential Applications in Periodontal Wound Healing – An In Vitro Study
Deepikha Krishnaraj, Kavitha Sanjeev, Thayalan Dineshkumar, Indumathi Nandhakumar, Amit ByatnalAbstract
Background:
Periodontal disease is a chronic inflammatory disorder, perpetuated by microbes and maintained by inflammatory responses of the host. Pleiotropic effects of herbal bioactives have immense scope as adjuncts in periodontal therapy.
Objective:
Assessment of the antimicrobial, anti-inflammatory, and antioxidant properties of fractionated bioactives from
Methods:
Tulsi leaves were extracted using five solvents of increasing polarity. The most active extract was fractionated by column chromatography, and three bioactive fractions were combined (1:1:1) to prepare a paste. Antibacterial, antioxidant, and anti-inflammatory activities were evaluated using agar well diffusion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and human red blood cell (HRBC) membrane stabilisation assays. Experiments were performed in triplicate, and data are presented as mean ± standard deviation (SD).
Results:
Tulsi fraction paste showed antibacterial activity against the tested periodontal pathogens, with greater efficacy against
Conclusion:
Fractionated bioactives of