Fe‐MOF‐NH 2 @Metformin as a Peroxidase‐Like Activity for Fluorometric Glucose Sensing
Padideh Sadeghi, Abdolraouf Samadi‐MaybodiABSTRACT
In this research, an amine‐containing metal–organic framework was modified with metformin (Fe‐MOF‐NH 2 @metformin) and used as peroxidase‐like activity for fluorometric determination of glucose. The best fluorescence emission intensity ( λ m = 450 nm) was found at the excitation wavelength of λ ex = 350 nm. Results indicated that H 2 O 2 and glucose can effectively quench the fluorescence emission of Fe‐MOF‐NH 2 @metformin, and the fluorescence intensity linearly decreased with increasing concentration of H 2 O 2 or glucose. Moreover, the quenching effect of H 2 O 2 is considerably enhanced in the presence of dopamine, and hence, this compound plays a role of synergistic effect in the quenching process, and accordingly, the spectra are more differentiated and more distinguishable. The Stern–Volmer quenching constant ( K SV ) for H 2 O 2 and glucose were obtained at 0.005 and 0.004 M −1 , respectively. The binding constant ( K b ) and the site number ( n ) of Fe‐MOF‐NH 2 @metformin were also investigated and discussed. The K b and n for glucose were obtained at 209 and 0.6839, likewise for H 2 O 2 , 77 and 0.5484, respectively. The detection limits for measuring glucose and H 2 O 2 were obtained 0.511 and 0.351 μM, respectively.