Exploring the Role of Wnt/β-Catenin Signaling in Gastric Cancer: Implications for Early Diagnosis and Targeted Therapy
Shaoqing DingBackground
Gastric cancer (GC), as one of the main causes of cancer-related death, has relatively few treatment options, and the Wnt/β-catenin signaling pathway plays a crucial role in the course of this disease; this study investigates the pathway’s role and evaluates the therapeutic potential of its inhibition using a rat model.
Materials and Methods
Fifty Sprague Dawley rats were divided into control, model, Wnt/β-catenin inhibition, IWR-1 treatment, and combined treatment groups, with GC induced by methyl-N′-nitro-N-nitrosoguanidine (MNNG) administration. Protein and mRNA expression levels of Wnt, β-catenin, and P-β-catenin were evaluated using Western blotting, hematoxylin–eosin (HE) staining, immunohistochemistry, and RT-PCR.
Results
The tumor proliferation of experimental rats can be significantly inhibited by suppressing the Wnt/β-catenin signaling pathway, and the expression levels of related molecules in this pathway also show obvious downregulation, with combined IWR-1 treatment further enhancing these therapeutic effects compared with the model group.
Conclusion
Targeting the Wnt/β-catenin signaling pathway shows good prospects as a potential strategy for the treatment of GC, with findings suggesting that its inhibition could limit tumor progression and warrant further investigation for clinical applications.