Experience of primary acclimatization of planting material in vitro Betula pendula var. Dalecarlica in the conditions of Arkhangelsk
Alina Krainova, Aleksandr Antonov, Ol'ga LebedevaThe article covers the experience of adaptation to natural conditions of the northern region of planting material Betula pendula var. Dalecarlica, which was obtained by laboratory micropropagation and transported to the place of the experiment. Dalecarlica birch is known for its decorative properties and resistance to the growing environment, so it has a high potential for use in landscaping northern cities. Plants cultivated in vitro provide genetic homogeneity and high quality of planting material. To minimize stress after transportation and preparation of seedlings for natural con-ditions, all plants were placed in the microclimate of a hydroponic installation for some time. Then, for acclimatization, the planting material was divided into three equal parts according to growing conditions: some remained in hydropon-ics, the rest were transferred to a greenhouse and open ground. The same substrate was used in all experimental condi-tions. For 40 days, monitoring of such basic morphometric parameters of plant growth and development as stem height, number of leaves and number of internodes was carried out. The highest indicators were observed in seedlings that were kept in hydroponic conditions. Other acclimatization environments played an additional stress factor for plants. The conducted studies demonstrated that optimal adaptation conditions contribute to more effective plant de-velopment. The results of this experiment can be useful for improving practices for adapting planting material to ex-vitro conditions of regions with a harsh climate in order to increase biodiversity and efficiency of plant cultivation, as well as for developing methods for improving the survival and development of cultures obtained in vitro.