DOI: 10.11648/j.ijcems.20261203.11 ISSN: 2469-8032

Evaluation of the Inhibitory Effect and Cytotoxicity of Some Nanoparticles Against Bacterial Isolates from Burn Infections

Asmaa Sultan
Show PDF Cite
150 swabs were taken from different burns of patients admitted to Medical City Hospital and Al-Kindi Teaching Hospital. 128 swabs showed morphological examinations. The isolates were confirmed at the species level using Vitek2 compact system for skin diseases. The isolates under study were tested for sensitivity using the drill diffusion method. Nanoparticles were prepared by two methods, chemical synthesis and green synthesis, Aloe vera and aqueous extract of saffron (Crocus sativus L.) . Bacteria were isolated by 60 isolates of Pseudomonas aeruginosa , (46.87%), followed by Staphylococcus aureus, with 38 isolates (12.28%), Klebsiella spp . with 20 isolates (15.62%), and the rest of the isolates were Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, Stenotrophomonas maltophilia , with three isolates for each type, i.e. (1.56 %). S. aureus and Ps. aeruginosa were the most resistant bacteria to antibiotics, and it was found that substance nanoparticle prepared by Artificial roads nanoparticle. The percentage of Staph. aureus bacteria was (28.12%), while Ps. aeruginosa bacteria was (46.87%). The solutions were at concentrations of (100, 75, 50, 25) M%. It is noted that the highest inhibition concentration was (100) M%. As for the is Staph. aureus and Ps. aeruginosa bacteria isolates zone (48, 45) mm, respectively. It was found that the saffron of (45) mm in inhibiting the isolates of Gram-positive bacteria Staph. aureus . The success of Staph. aureus with nickel oxide with saffron at a rate of MIC. Invivo test animals, and the MBC the conducted two methods tests were, ZETA POTENTIAL. Nanomaterials with a smaller size of 5-12nm called quantum dot were observed, and no agglomeration occurred in them. It was shown that the MTT toxicity assay for the solutions of the IC50 ratio for cancerous PC3, while for cellsand the substance is not toxic to the solution for treating S. aureus bacteria, and Ps. aeruginosa.

More from our Archive