DOI: 10.4103/jcde.jcde_314_26 ISSN: 2950-4716

Evaluation of cytotoxicity, setting time, and mineralization potential of mineral trioxide aggregate with Nigella sativa aqueous extract: An in vitro study

Shanmugapriya Karunakaran, K. E. Selvendran, Hafeela Farhana, Ashok Leburu, Madhuram Krishnamurthy
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Abstract

Aim:

To evaluate the effect of different concentrations of Nigella sativa (NS) aqueous extract on the cytotoxicity, setting time, and mineralization potential of mineral trioxide aggregate (MTA).

Materials and Methods:

In this in vitro study, three groups were evaluated: MTA + 30% NS (Group A), MTA + 50% NS (Group B), and MTA alone (Group C). Cytotoxicity was assessed using the MTT assay on MG-63 osteoblast-like cells. Setting time was measured using the Gillmore needle test according to the ISO 6876 standards. Mineralization was evaluated using Alizarin Red S staining. Statistical analysis was performed using one-way analysis of variance and Tukey’s post hoc test ( P < 0.05).

Results:

The addition of NS improved cell viability in a concentration-dependent manner, with Group B showing the highest viability. Both NS groups demonstrated reduced initial and final setting times compared to MTA alone. Mineralization was significantly enhanced in NS groups, particularly in Group B.

Conclusion:

Incorporation of NS, especially at 50%, enhances the biocompatibility, accelerates the setting time, and improves the mineralization potential of MTA, suggesting its potential as a beneficial modification for endodontic applications.

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