Evaluating the impact of blue-filtering intraocular lenses on macular oxidative stress: A comparative analysis of 8-hydroxy-2’-deoxyguanosine levels in the retinal tissue of donor eyes
Karvannan Sevugamurthi, Padmapriya Sivashanmugam, Sujay Jaju, P. S. Janani, Vijayakrishna Sakthivel, Navaneeth Kumar, Vignesh Elamurugan, Jaishree Pandian, Sankha Amarakoon, Tos T. J. M. Berendschot, Siddharth NarendranPurpose:
To evaluate the impact of blue-filtering intraocular lenses (BFIOLs) on retinal oxidative stress by comparing 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels in the macular retina and submacular retinal pigment epithelium (RPE) of donor eyes with BFIOLs and non-BFIOLs.
Design:
Cross-sectional laboratory study using postmortem human donor eyes.
Methods:
Fifty-eight eyes from 39 donors were categorized as BFIOL (n = 16), non-BFIOL (n = 24), or phakic (n = 18). Macular retina and submacular RPE were dissected, genomic DNA extracted, and 8-OHdG quantified using ELISA. 8-OHdG levels were compared across lens groups, and associations with age and sex were examined.
Results:
Submacular RPE had higher 8-OHdG levels than the macular retina (0.95 vs 0.44 ng/mL;
Conclusions:
Macular retinal and RPE 8-OHdG levels did not differ significantly by IOL type. These biochemical observations, considered alongside existing clinical data, suggest that any retinal protective effect of BFIOLs on oxidative DNA damage is likely to be modest.