Estimation of IFN-γ, IL-4, and IL-17 in Saliva of Lichen Planus Patients
Safaa Moaid Asker, Maha Adel Mahmood, Lateef Essa AlwanAbstract
Background:
One of the most prevalent T cell-mediated skin and mucous membrane illnesses includes lichen planus (LP). Several cytokines have been found in LP lesions and patient sera, supporting the idea that LP is an inflammatory skin disease.
Objective:
The current study aims to measure the salivary level of IFN-
Materials and Methods:
Saliva collection was done by using the method of Wu-Wang, as saliva samples were collected at a specific time in the early morning 9 AM to 12 PM, and instruction was given that no food or liquids, other than water, should be consumed by the patient for at least 1 h before saliva collection. All individuals were instructed to spit saliva for 5 min without swallowing into a clean plastic container, which was kept in ice during the collection. Then, samples were centrifuged, and the supernatant was stored at –70°C until immunological analysis by ELISA was done.
Results:
The results demonstrate that highly significant differences are registered among the patients groups regarding “IFN-
Conclusion:
It could be concluded that “IFN-