Efficiency of Synthetic Hormone for Successful Induced Breeding of Mystus Tengara

An experiment was conducted to determine the optimum hormone dose for the induced breeding of the commercially important Mystus tengara. Juvenile of M tenrara (weighing approximately 8 g) were reared for seven months (February-August) and fed with a diet containing 45% protein while maintaining optimal water quality throughout the experiment. Nine treatments were designed using different hormone dilutions prepared with physiological saline (0.9% NaCl). The commercially available breeding hormone Spawn Pro, containing salmon gonadotropin-releasing hormone analogue (sGnRHa) and domperidone, was administered via intraperitoneal injection to the brood fish. The gonadosomatic index (GSI) and hepatosomatic index (HSI) showed gradual changes over the reproductive cycle. In males and females, GSI increased moderately from May to June, reaching a peak value of 5.56 ± 0.15% and 18.31 ± 0.04% during June. Female HSI exhibited a different trend, with the highest value recorded in May followed by a significant decline in June, indicating mobilization of energy reserves during gonadal maturation. Among the treatments, Treatment J (intermediate hormone dose with 7 ml saline dilution) was found to be statistically superior in maximizing reproductive performances. It produced the highest egg yield (4873.67 ± 124.97), which was significantly greater than any other treatments. The treatment J also achieved the highest fertilization success rate (90.00% ± 0.00), which was significantly superior to other treatments. These findings indicated that an intermediate hormone dose, combined with an appropriate saline dilution, was most effective for maximizing ovulation and fertilization of M. tengara. The study provides a standardized protocol for induced breeding, supporting large-scale seed production and promoting species diversification in aquaculture systems.