DOI: 10.31594/commagene.1747173 ISSN: 2602-456X

Effect of Potassium Nitrate on Growth Rate, Growth Kinetics, Metabolites, and Lipid Profile of Euglena sp. Isolated from the Dieng Plateau

Fitri Natalia, Renata Adaranyssa Egistha Putri, Renisha Windy Puspita Sari, Eko Agus Suyono
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Euglena sp. can absorb high levels of CO2 and contains valuable compounds whose production can be enhanced by KNO₃ supplementation. This study aimed to determine the effect of KNO3 on the growth rate and to identify the most suitable non-linear model for population growth, biomass, carbohydrate, protein, pigment, α-tocopherol content, lipid concentration, and lipid profile of Euglena sp. cultured with 15% CO₂. Treatments consisted of KNO₃ concentrations of 0.25, 0.5, and 0.75 g/L as well as positive and negative controls (0.0 g/L). The results showed that the addition of 0.5 g/L KNO₃ produced the highest growth rate and the greatest biomass, carbohydrate, protein, chlorophyll-b, carotene, and lipid contents with respective values of 8.4 × 10⁶ cells/mL (cell density), 0.6 ± 0.01 g/L, 4.34 ± 0.055 g/L, 10.68 ± 0.38 × 10⁻³ g/L, 2.19 ± 0.13 µg/mL, and 0.25 ± 0.07 g/L. The most suitable growth model was the Gompertz model, which showed an R² value greater than 0.9. The highest chlorophyll-a content was observed in the negative control (3.75 ± 0.00 µg/mL), while the highest α-tocopherol content occurred with 0.75 g/L KNO₃ (95 ± 0.00 µg/mL). The 0.5 g/L KNO₃ treatment also produced the broadest range of fatty acids, with 16 types identified.

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