DOI: 10.1177/1934578x261463594 ISSN: 1934-578X

Discovery of a Cryptic Secondary Metabolite, Plantazine, From the Mesophilic Plant Pathogen Burkholderia plantarii

Yeo Jin Lee, Eun Seo Jang, Hyeon-Jeong Hwang, Youngsang Nam, Tae Gyun Kim, Chaeyun Hong, Eun La Kim, Mohammad R. Seyedsayamdost, Seoung Rak Lee
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Objectives

Cryptic secondary metabolites from bacteria mostly remain undetected under standard cultivation conditions because their biosynthetic gene clusters are not expressed or are only weakly expressed. The present study aimed to discover cryptic secondary metabolites from Burkholderia plantarii using a high-throughput elicitor screening (HiTES) platform.

Methods

HiTES was applied to activate cryptic biosynthetic gene clusters in B. plantarii . Culture extracts derived from elicitor-treated conditions were subjected to repeated column chromatography for compound isolation. The chemical structures of the isolated compounds were elucidated using a combination of 1D and 2D NMR spectroscopic experiments, HR/MS data, and Marfey`s method. All isolated compounds were evaluated for their antibacterial and antifungal activities against pathogenic bacterial and yeast strains.

Results

In the HiTES approach, a cryptic secondary metabolite, plantazine ( 1 ), with three known compounds ( 2 - 4 ) was isolated and structurally identified from B . plantarii . Comprehensive spectroscopic analyses conclusively determined the complete structure and stereochemistry of plantazine.

Conclusions

HiTES represents an efficient, comprehensive, and broadly applicable approach for the discovery of cryptic secondary metabolites by inducing the expression of silent biosynthetic gene clusters from bacteria.

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