DOI: 10.1039/d6cb00180g ISSN: 2633-0679
Discovery and evolution of an ( R )-selective transaminase for production of sitagliptin
Matthew Treadell, Gareth R. E. Surman, Grayson J. Ford, Christopher W. Otun, Shiny Joseph Srinivasan, James Galman, Maria Bawn, Akash Pandya, Craig J. Markin, Anthony P. Green, Jack S. RowbothamA new ( R )-selective transaminase (RTA-223, UniProt W9Z089) is identified from Capronia coronata with activity towards a range of bulky ketone substrates. We demonstrate the directed evolution of the enzyme for production of the challenging pharmaceutical target ( R )-sitagliptin.