DOI: 10.1128/mra.00811-23 ISSN: 2576-098X
Direct metagenomic and amplicon-based Nanopore sequencing of French human monkeypox from clinical specimen
Aurelia Kwasiborski, Véronique Hourdel, Charlotte Balière, Damien Hoinard, Quentin Grassin, Maxence Feher, Clémentine De La Porte Des Vaux, Mélanie Cresta, Jessica Vanhomwegen, Jean-Claude Manuguerra, Christophe Batéjat, Valérie Caro- Genetics
- Immunology and Microbiology (miscellaneous)
- Molecular Biology
ABSTRACT
We report the whole-genome sequence of monkeypox virus obtained using MinION technology (Oxford Nanopore Technologies) from a French clinical specimen during the 2022 epidemic. Amplicon-based sequencing and shotgun metagenomic approaches were directly applied to the sample.