Dielectric Elastomer Actuators as Safe and Effective Tools for Mechanostimulation of Human Cells

Mechanical forces are central to regulating cell health, yet reproducing such forces in vitro remains challenging. Existing systems are bulky, limited in design, or unsuitable for long‐term use. Dielectric elastomer actuators (DEAs) offer a soft and flexible alternative capable of delivering controlled mechanical strain, but concerns remain that the high voltages required for actuation might harm cells through electric field exposure. Here, we systematically evaluate DEA‐based cell stretchers using human bladder cell line TEU‐2, comparing them with a commercial motor‐driven stretcher and a nondeforming dielectric polymer actuator that isolates electrical from mechanical effects. Across multiple assays, DEA stimulation preserved cell viability and morphology without inducing DNA damage, apoptosis, necrosis, or cell‐cycle disruption. Furthermore, comet assays, flow cytometry, and quantitative gene expression analysis confirmed the absence of genotoxicity and stress responses. Instead, DEA actuation triggered transcriptional changes consistent with physiological mechanotransduction, highlighting its functional relevance. Our findings establish DEAs as a safe and effective platform for long‐term mechanostimulation. While demonstrated in bladder‐derived cells, the approach is broadly applicable to diverse biological models where mechanical cues are critical. This work expands the technological foundation for physiologically relevant in vitro systems, supporting advances in tissue engineering, disease modeling, and personalized medicine.