Diagnostic and Prognostic Value of
miR
‐27a‐5p in Childhood Severe Pneumonia Through Targeted Regulation of
FOXO1
Yupei Liu, Haobin Wang, Jianzhu He, Ludan Deng, Xiao Zhou ABSTRACT
Severe pneumonia in children deteriorates rapidly and is very destructive. miR‐27a‐5p has been shown to function in multiple cancers and inflammatory conditions, but its role in severe pneumonia is unknown. To investigate the diagnostic and prognostic value of miR‐27a‐5p for severe pneumonia in children. This study included 146 children with severe pneumonia as subjects and 123 children with mild pneumonia as controls. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) was employed to assay miR‐27a‐5p and FOXO1 levels. ROC and Kaplan–Meier curves were employed to assess the diagnostic and prognostic values. Cell viability and apoptosis were determined by cell counting kit‐8 (CCK‐8) and flow cytometry. The concentrations of tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), and IL‐1β were measured using enzyme‐linked immunosorbent assay. Luciferase reporter assay for miR‐27a‐5p targeting FOXO1. miR‐27a‐5p level was higher in severe pneumonia. miR‐27a‐5p has a good diagnostic and prognostic value for severe pneumonia. miR‐27a‐5p down‐regulation promotes cell viability and inhibits apoptosis and inflammatory factor production. FOXO1 is a target of miR‐27a‐5p. And FOXO1 may reverse the effects of miR‐27a‐5p on the biological function of HFL1 cell. In conclusion, miR‐27a‐5p may be a potential pneumonia marker by negatively regulating FOXO1 to worsen pneumonia.