DOI: 10.4103/mjbl.mjbl_266_23 ISSN: 1812-156X

Detection of Some Resistance Genes and Biofilm Production in Methicillin-Resistant Staphylococcus aureus Isolates from Clinical Sources

Fatima Salem Obaid Al Jasmi, Ali Abdul Kadhim Al-Ghanimi
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Abstracts

Background:

Hospitals around the world are currently suffering from Methicillin-resistant Staphylococcus aureus (MRSA) as a major pathogen in hospitals.

Objectives:

Methicillin-resistant Staphylococcus aureus infections are caused by decades of unneeded use of antibiotics. Antibiotics have been prescribed for years to treat colds and influenza in cases of viral infections that do not respond to these drugs, and thus increase resistance in staphylococci, which in turn leads to very serious and potentially fatal diseases.

Materials and Methods:

From the beginning of December 2021 until the end of March 2022, samples of wounds, burns, blood, skin, and urine were collected from several hospitals in Kerbala, Iraq. A total of 150 samples were cultured immediately after collection for diagnosis.

Results:

The Staphylococcal isolates were identified through microscopic and biochemical examinations using the analytical profile index 20E system. The isolation process resulted in obtaining 31 S. aureus isolates, which were subsequently tested for their resistance to antibiotics. Finally, investigated methicillin and tetracyclines genes and the ability of these bacteria to produce biofilms by qualitative and quantitative methods.

Conclusion:

An increase in the proportions of MRSA was observed in the holy city of kerbala, and all MRSA bacteria produced biofilms with varying in strength.

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