Detection of Serotonin Gene Hormone (HTR2A)-28 G/A Polymorphism in Autistic Children in Middle Provinces, Iraq
Azal A. Al-Rubaeaee, Ali Hadi Mahdi, Russul Feihan MussaAbstract
Background:
Autism is a common neurodevelopmental disorder of childhood. The majority of patients with autism spectrum disorder (ASD) are male, with a male-to-female ratio of 4:1. Serotonin is a monoamine neurotransmitter that plays a crucial role in the neurodevelopmental stage. Additionally, polymorphism in the serotonin 2A receptor gene has been shown to be associated with autism.
Objectives:
To find out whether there is a link between the HTR2A gene and autism in children.
Materials and Methods:
DNA was extracted from the peripheral blood of 50 autistic patients and 50 healthy people to see if there was a link. The serotonin gene was genotyped using the PCR- restriction fragment length polymorphism (RFLP) technique. The data was examined using the odds ratio with 95% confidence intervals and the Chi square (χ²) results have been statistically verified. A
Results:
The allelic frequency of the gene was 94.5% in the control group and 70.7% in the autistic sets, with significant variances. Homozygous AA (43.4%) and homozygous GG (32.3%) were more prevalent than the heterozygous (32.3), with significant variances between them (33.3%).
Conclusion:
There is a link between autism and mutations in the HTR2A gene.