Dharam Bai Meena, Geeta Parihar, Swati Singh Shekhawat

DETECTION OF METALLO-β-LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA IN HOSPITALIZED & OUTDOOR PATIENTS AT A TERTIARY CARE HOSPITAL IN WESTERN RAJASTHAN.

  • General Medicine
  • General Earth and Planetary Sciences
  • General Environmental Science
  • General Medicine
  • Ocean Engineering
  • General Medicine
  • General Medicine
  • General Medicine
  • General Medicine
  • General Earth and Planetary Sciences
  • General Environmental Science
  • General Medicine
Save to CiteDrive Show PDF

Background: Pseudomonas aeruginosa is emerging as a nosocomial pathogen by producing metallo-β-lactamases (MBLs) and thus, inicting signicant morbidity and mortality worldwide. Aim:This study aimed to compare three phenotypic methods to detect MBL production in Pseudomonas aeruginosa and to determine the antibiotic sensitivity of MBL-producing isolates. A total of 100 non-repet Material and Methods: itive P. aeruginosa isolates from various clinical specimens between July 2012 and August 2013 were tested for MBL production. Pseudomonas aeruginosa strains were detected by conventional phenotypic identication scheme. Imipenem and Ceftazidime resistant isolates were selected for the detection of MBL production by three phenotypic methods 1) Combined disk test (CDT). 2) Double disk synergy test (DDST) and 3) Modied Hodge test (MHT). Out of 100 Pseudomonas Results: aeruginosa strains, 15 resistant to Imipenem and Ceftazidime were taken as screening positive. Out of 15, 14(93.33%) isolates were MBL positive by CDT methods, 10(66.66%) by DDST method and 6(40%) by MHT respectively. The most effective antibiotic against Pseudomonas aeruginosa was Colistin. The study result suggests that early detect Conclusion: ion of MBL-producing P. aeruginosa and determination of their antibiotic sensitivity are of crucial importance to start appropriate treatment. It was observed in present study that Combined disk test (CDT) was most effective for MBL detection.

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

  • Web-based, modern reference management
  • Collaborate and share with fellow researchers
  • Integration with Overleaf
  • Comprehensive BibTeX/BibLaTeX support
  • Save articles and websites directly from your browser
  • Search for new articles from a database of tens of millions of references
Try out CiteDrive

More from our Archive