DOI: 10.4103/mjbl.mjbl_105_24 ISSN: 1812-156X

Detection Mitochondrial tRNAAla Encoding Gene Polymorphism in Colorectal Cancer Patients

Roaa Hammed Alwaidh, Ali Fadhil Hashim, Mona N. Al-Terehi, Liwaa Hussein Mahdi
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Abstract

Background:

The transfer RNA is the focus of attention of researchers recently due to its role in cell functions. Variations in tRNA-encoding gene, modifications and levels have been observed in different diseases.

Objectives:

The current study was proposed to detect some single nucleotide polymorphisms (SNPs) in tRNA Ala -encoding gene in mitochondria of colorectal cancer patients.

Materials and Methods:

A case–control design included 45 colorectal cancer patients with different cancer stages and 30 healthy individuals as the control group, DNA extraction, modified nested PCR and whole tRNA Ala -encoding gene sequencing were used to detect variations.

Results:

The G/A rs786200950, A/G rs1603220045 (C in the present study) were observed in the patient group only (OR = 2.4783, CI 95% 0.0972–63.2090, P < 0.5829), while C/T rs879226228, A/G rs878963919 and A/G rs2068695009 were found in the control group only (OR = 0.2582, CI 95% 0.0101–6.5877, P < 0.4126). Other non-polymorphic SNPs in the study groups had (OR = 0.8028, CI 95%0.0154 –41.7311, P < 0.9132). According to the type of CRC which included adenocarcinoma and mucinous adenocarcinoma no significant association was observed. Also, two SNPs (Grs786200950A and A rs1603220045 G (C)) were observed in adenocarcinoma (OR 0.8182, CI 95% 0.0301–22.2038, P < 0.2582). In the present study, eight SNPs were reported. Five of them were reported SNPs (have rs numbers in NCBI), while three were unreported SNPs. Each SNP was found in a single observation.

Conclusion:

The present findings concluded that there were low variations in tRNA Ala in colorectal cancer patients. Non-association with colorectal cancer subtypes.

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