Description of Corynebacterium nasorum sp. nov. and Corynebacterium hallucis sp. nov. isolated from human nasal passages and skin
Elena B. Popowitch, Tommy H. Tran, Isabel F. Escapa, Eeshta Bhatt, Alp K. Sozat, Nashwa Ahmed, Clayton Deming, Ari Q. Roberts, NISC Comparative Sequencing Program, Julia A. Segre, Heidi H. Kong, Sean Conlan, Katherine P. Lemon, Matthew S. KellyStrains of two novel Corynebacterium species were cultured from samples of human nostrils and skin collected in the USA and Botswana. These strains demonstrated growth on Columbia Colistin-Nalidixic Acid agar with 5% sheep blood and in liquid media (brain–heart infusion and tryptic soy broth) supplemented with Tween 80, a source of the fatty acid oleic acid. Cells were Gram-positive, non-spore-forming, non-motile bacilli that showed catalase but not oxidase activity. Major fatty acids in both of these species were C 18 : 1 ω9c (oleic acid), C 16 : 0 (palmitic acid) and C 18 : 0 (stearic acid). Analysis of the 16S rRNA gene sequences identified these strains as belonging to the genus Corynebacterium (family Corynebacteriaceae ). Whole-genome sequencing revealed that these strains formed distinct branches on a phylogenomic tree, with average nt identities of <95% relative to all previously described species. These results indicate that these strains represent novel species of Corynebacterium , for which we propose the names Corynebacterium hallucis sp. nov., with the type strain CTNIH22 T (=ATCC TSD-435 T =DSM 117774 T ), and Corynebacterium nasorum sp. nov., with the type strain KPL3804 T (=ATCC TSD-439 T =DSM 117767 T ). These data also indicate that ‘ Corynebacterium kefirresidentii ’ is designated C. nasorum sp. nov.