Dentinal tubule penetration of a bioceramic sealer after different irrigant and activation regimens: An in vitro confocal laser scanning microscopy study
Nalluri Nikhita, TBVG Raju, Krishna Prasad Parvathaneni, Abitha Seshadri, Gowtam Dev Dondapati, Shikha SharmaAbstract
Background:
Proper sealer adaptation relies on effective smear layer removal and adequate irrigant penetration. Intratubular entry of sealers is influenced by both the irrigant used and the method of activation. Nevertheless, evidence regarding the impact of newer chelators and activation strategies on bioceramic sealer penetration remains limited.
Aim:
This study aimed to assess and compare how various irrigating solutions and activation techniques influence the extent to which a bioceramic sealer penetrates the dentinal tubules, as observed through confocal laser scanning microscopy (CLSM).
Materials and Methods:
Sixty-three single-rooted teeth were prepared to F3 and randomly assigned to three activation groups (
Statistical Analysis:
Data were examined with one-way ANOVA and Tukey’s post hoc test at a significance level of
Results:
Diode laser activation demonstrated significantly greater sealer penetration than sonic and passive ultrasonic irrigation (PUI) techniques (
Conclusion:
Both irrigant type and activation method significantly influenced bioceramic sealer penetration. Diode laser activation was the most effective, while EDTA remained superior to HEDP and chitosan according to this study.