Construction of Injectable Hydrogels Based on Cationic Porphyrins and Hyaluronic Acid for Wound Healing With Multimodal Antibacterial Therapy and Instantaneous Monitoring
Penghan Yue, Yiding Wang, Cong Liu, Hongqian Liu, Yongjie Lin, Mingzhong Li, Liujun Xu, Yujia Huang, Yanhui Li, Huayu TianABSTRACT
Although hydrogels with antimicrobial and monitoring have the potential for precision wound therapy, it is difficult to construct smart framework of hydrogels in molecular level. Herein, an in situ crosslinked antimicrobial conductive hydrogel (Au/HA‐TPP) based on 5,10,15,20‐tetra(4‐ N ‐(4‐boronobenzyl)pyridin) porphyrin (TBPP) and hyaluronic acid (HA) was prepared triggered by an alkaline solution containing Au(III) reduction catalysis with phenylboronic acid, (5,10,15,20‐tetra(4‐dihydroxyborylphenyl) porphyrin (TBP) and 5,10,15,20‐tetrakis‐(4‐N‐methylpyridyl) porphyrin (TMPyP)) as control. In the process of gelation, Au(III) was reduced into Au nanoparticles (Au NPs) and formed into nanoparticles and dispersed evenly in Au/HA‐TPP with fastest gelation time of 22 s and the best mechanical properties. The synergistic interaction of Au NPs and porphyrins can induce effective antibacterial photodynamic therapy (APDT) under visible light irradiation, and the photothermal effect caused by Au NPs had a fast temperature rise in seconds with good cycling stability. In addition, quaternized porphyrins in Au/HA‐TPP played a long‐term antibacterial and APDT effects. The dynamic change of the contact resistance between the hydrogel and wounds can reflect the wound healing to achieve instantaneous monitoring. Au/HA‐TPP hydrogels integrated multimodal sterilizations as well as the realization of intelligent wound detection, which could provide the diagnosis and treatment of infected wounds at different application scenarios.