Constant enzymatic <i>in situ</i> production of H₂O₂ for an Unspecific Peroxygenase by an L‐amino acid oxidase

Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H₂O₂) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H₂O₂. In order to prevent the deactivation, we developed an enzyme cascade with an L‐amino acid oxidase (LAAO) to produce hydrogen peroxide <i>in situ</i> at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95 %.