DOI: 10.1128/mra.00507-25 ISSN: 2576-098X

Complete genome annotation of the cluster EC Microbacterium phage Nicole72 from Pittsburgh, Pennsylvania

Adam J. Ziegler, Christa P. Joby, Hams A. Kamil, Ethan Johnson, Ashlyn L. Ivey, Vanessa A. Fullante, Gautam Ghosh, Laura Leal Martinez, Gabrielle A. Tutelo, Devya Wilson, Christine A. Byrum

ABSTRACT

Nicole72, a microbacteriophage isolated from a flowerbed in Pittsburgh, Pennsylvania, has a 55,431 bp genome containing 90 predicted protein-coding genes and no transfer RNAs or transfer-messenger RNAs. Nicole72 has siphovirus morphology and is most closely related to the EC cluster microbacteriophage Megan where BLASTn indicates 81.5% identity, 86% query coverage.

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