Comparison of the effect of training under normal conditions and normobaric hypoxia on changes in blood serum using differential scanning calorimetry (DSC)
Klaudia Duch, Michał Krzysztofik, Karolina Rykała, Ewa Sadowska-KrępaAbstract
Introduction
The aim of this study was to investigate the acute effects of a single leg press resistance training session, performed at different intensities and under different conditions (normoxia and normobaric hypoxia), on blood serum proteins assessed by differential scanning calorimetry (DSC).
Material and methods
Fourteen resistance-trained men participated in the trial. Each subject completed three sessions: one familiarization session and two experimental sessions separated by one week. All sessions were conducted at the same time of day (9:00–11:00) to control for circadian influences. Blood samples were collected at rest (baseline, BA) and immediately post-exercise (POST). Serum samples were analyzed using DSC.
Results
DSC profiles revealed post-exercise changes in serum protein denaturation. Statistically significant differences were observed between pre- and post-exercise states for parameters of the endothermic transition associated with protein denaturation.
Conclusions
The findings demonstrate the feasibility of using DSC to monitor the effects of exercise performed at different intensities and under varying oxygen conditions (normoxia and normobaric hypoxia) on serum protein changes.