Comparative Analysis of Analytical Techniques for Quantification of Valsartan and Sacubitril in Pharmaceutical Preparations – A Review

Accurate measurement of Valsartan and Sacubitril in pharmaceutical formulations is crucial for ensuring both therapeutic efficacy and safety in heart failure treatment. This review compares three analytical techniques—Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), UV spectrophotometry, and LC-MS/MS—aiming to identify the most effective method for drug quantification. RP-HPLC emerges as the most suitable method for routine analysis due to its superior resolution, precision, and reproducibility. In contrast, UV spectrophotometry serves as a simpler, cost-effective solution for bulk drug testing in large batches, offering reduced complexity but with less sensitivity. Advanced techniques such as LC-MS/MS and spectrofluorimetric analysis deliver exceptional sensitivity, making them ideal for pharmacokinetic studies; however, they are less practical for everyday testing. Furthermore, green chemistry initiatives are gaining traction as they minimize environmental impact while maintaining high analytical accuracy. Although RP-HPLC remains the preferred method, the evolving landscape of analytical techniques, coupled with sustainable practices, is reshaping the future of pharmaceutical quality control.