Codon Usage Analysis of Aminnopeptidase N Gene in Order Diptera

Aminopeptidase N (APN) has been identified as Cry toxin-binding proteins in various insects and also responsible for digestion of peptides. An attempt has been made on the APN orthologous to overview the genome-wide analysis of APN gene in Order Diptera. The entire ORF of APN gene sequences belonging to mosquito and non-mosquito species in Order Diptera have been retrieved from NCBI and analysed by various bioinformatics tools. The goal of this study is to perform comparative analysis of APN nucleotide sequences, nucleotide composition, codon usage bias pattern, amino acid composition bias, pattern of conservation among amino acid residues, and phylogenetic analysis among insect species belonging to the order Diptera. Our results may provide useful insights on the patterns of codon usage bias that facilitate a better understanding of the structure and evolution of gene coding sequences of these insects