Cell-type composition affects adipose gene expression associations with cardiometabolic traits
Sarah M. Brotman, Anniina Oravilahti, Jonathan D. Rosen, Marcus Alvarez, Sini Heinonen, Birgitta van der Kolk, Lilian Fernandes Silva, Hannah J. Perrin, Swarooparani Vadlamudi, Cortney Pylant, Sonia Deochand, Patricia V. Basta, Jordan M. Valone, Morgan N. Narain, Heather M. Stringham, Michael Boehnke, Johanna Kuusisto, Michael I. Love, Kirsi H. Pietiläinen, Päivi Pajukanta, Markku Laakso, Karen L. Mohlke- Endocrinology, Diabetes and Metabolism
- Internal Medicine
Understanding differences in adipose gene expression between individuals with different levels of clinical traits may reveal the genes and mechanisms leading to cardiometabolic diseases. However, adipose is a heterogeneous tissue. To account for cell-type heterogeneity, we estimated cell-type proportions in 859 subcutaneous adipose tissue samples with bulk RNA-sequencing (RNA-seq) using a reference single nuclear RNA-seq dataset. Cell-type proportions were associated with cardiometabolic traits; for example, higher macrophage and adipocyte proportions were associated with higher and lower body mass index (BMI), respectively. We evaluated cell-type proportions and BMI as covariates in tests of association between >25,000 gene expression levels and 22 cardiometabolic traits. For >95% of genes, the optimal, or best fit, models included BMI as a covariate, and for 79% of associations, the optimal models also included cell type. After adjusting for the optimal covariates, we identified 2,664 significant associations (P ≤ 2e-6) for 1,252 genes and 14 traits. Among genes proposed to affect cardiometabolic traits based on colocalized genome-wide association study and adipose expression quantitative trait locus signals, 25 showed a corresponding association between trait and gene expression levels. Overall, these results suggest the importance of modeling cell-type proportion when identifying gene expression associations with cardiometabolic traits.