DOI: 10.1093/bioinformatics/btv351 ISSN:

BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Felipe A. Simão, Robert M. Waterhouse, Panagiotis Ioannidis, Evgenia V. Kriventseva, Evgeny M. Zdobnov
  • Computational Mathematics
  • Computational Theory and Mathematics
  • Computer Science Applications
  • Molecular Biology
  • Biochemistry
  • Statistics and Probability
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Abstract

Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.

Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.

Availability and implementation: Software implemented in Python and datasets available for download from http://busco.ezlab.org.

Contact:  evgeny.zdobnov@unige.ch

Supplementary information:  Supplementary data are available at Bioinformatics online.

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