BSA‐Stabilized Silver Nanoclusters Enhanced Fluorescence of a Dicyanoisophorone Derivative With o‐ Vanillin for Sensitive Detection of Hydrogen Sulfide (H 2
Rajanee Nakum, Suban K SahooABSTRACT
The endogenous hydrogen sulfide (H 2 S) is a vital signaling molecule and plays an important role in the physiological processes. Its abnormal level can cause various detrimental effects. Thus, there is a bourgeoning need to develop facile and sensitive methods for detecting H 2 S in various biological samples. Herein, a fluorescence‐based sensing platform has been developed for the ultrasensitive detection of H 2 S using an aggregation‐induced emission (AIE) active dicyanoisophorone derivative with o‐ vanillin (DSVL). AIE luminogen (AIEgen) fluorescence emission at 530 nm was quenched selectively by H 2 S, which enables its detection with a detection limit of 3.88 µM. Interestingly, when the probe DSVL was interacted with bovine serum albumin‐stabilized silver nanoclusters (BSA‐AgNCs), the formation of nanocomposite BSA‐AgNCs_DSVL resulted a significant enhancement in the relative quantum yield of DSVL from 31.88% to 83.84%. The highly fluorescent nanocomposite BSA‐AgNCs_DSVL showed an improved sensitivity with the detection limit down to 0.86 nM. Finally, the analytical utility was examined by detecting H 2 S in real samples, such as blood serum and vegetable extracts.