BODIPYs as Fluorogenic Probes for Live-Cell Imaging of Lipid Droplets: Photophysical and Computational Studies
Luana A. Machado, Cynthia L. M. Pereira, Ana Clara G. de Souza, Elivelton A. Ferreira, Flávia F. C. Marques, Ednilsom Orestes, Maria H. Araujo, Karen L. R. Paiva, Marina S. Mesquita, Sônia N. Báo, Leandro F. Pedrosa, Muhammad Jawad Nasim, Claus Jacob, Eufrânio N. da SilvaJúnior- Organic Chemistry
- Biochemistry
Introduction:
Lipid droplets (LDs) serve as primary storage sites for neutral lipids within cells and are crucial for lipid metabolism. Disorders affecting LDs can contribute to the pathogenesis of common metabolic diseases such as obesity and can-cer, highlighting the importance of comprehending LD biology in health and disease contexts.
background:
Several synthetic methods for the preparation of BODIPYs are reported in the literature, yet the exploration of the different applications of this exciting class of fluorescent compounds is less studied and, therefore, a wide avenue of possibilities remains to be discovered.
Methods:
Fluorescence assays are commonly used for the detection and quantification of lipids in biological samples or lipid-rich environments. In this study, BODIPYs were synthesized and analyzed for structural confirmation.
objective:
synthesis of BODIPYs and evaluation of their affinity for intracellular lipid droplets
Results:
These compounds were subsequently evaluated for photophysical, electro-chemical (cyclic voltammetry) and theoretical analysis, followed by live-cell imaging studies to confirm their affinity for intracellular lipid droplets.
method:
In this study, BODIPYs were synthesized and analyzed for structural confirmation. These compounds were subsequently evaluated for photophysical, electrochemical (cyclic voltammetry) and theoretical analysis followed by live-cell imaging studies to confirm their affinity for intracellular lipid droplets.
Conclusion:
BODIPYs have been identified as fluorogenic probes for live-cell imag-ing studies and found to serve as efficient and selective fluorescent compounds for intracellular lipid droplets.
result:
BODIPYs have been identified as fluorogenic probes for live-cell imaging studies and found to serve as efficient and selective fluorescent compounds for intracellular lipid droplets.