Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop
Lucia Paolini, Selene Tassoni, Khuloud T. Al‐Jamal, Xavier Banquy, Tamás Beke‐Somfai, Paolo Bergese, Fanni Annamária Boros, Massimo Bottini, Ben Boyd, Sara Busatto, Benedetta Bussolati, Ester Canepa, Valentina Castagnola, Aled Clayton, Kenneth Dawson, Samir El‐Andaloussi, András Försönits, Orazio Fortunato, Roberto Frigerio, Martina Gabrielli, Cristina Grange, Michele Guescini, Yuya Hayashi, Jonas Klejs Hemmingsen, Seungmin Kim, Ilyes Kinga, Heikki Kyykallio, Metka Lenassi, Luize Goncalves Lima, Mỹ G. Mahoney, Camille Menaceur Vandenbroucke, Andreas Möller, Angelo Musicò, Inge Nelissen, Lucas Fabrício Bahia Nogueira, Christa Nöhammer, Pietro Parisse, Charles Ramassamy, Kelly Cristine Santos Roballo, Anna Salvati, María Sancho‐Albero, Ganesh Shelke, Paniz Shirmast, Tasvilla Sonallya, Imola Csilla Szigyártó, Monique A. J. van Eijndhoven, Tapani Viitala, Shan Wang, Yan Yan, Andrea Zendrini, Edit Buzás, Annalisa RadeghieriABSTRACT
The concept of the “biomolecular corona” (BC) was first introduced to describe the spontaneous absorption of molecules, mainly proteins, onto the surface of synthetic nanoparticles upon exposure to biological fluids. More recently, the same term has been used to describe biomolecules that dynamically interact with the surface of extracellular vesicles (EVs) during their biogenesis, secretion, or upon exposure to intracellular or extracellular milieus, including biological fluids. The EV biomolecular corona is a key determinant of EV identity, shaping their cellular uptake, biodistribution, and functional outcomes in both physiological and pathological contexts. EVs, in contrast to synthetic nanoparticles, introduce distinct challenges and perspectives due to their biological origin, innate heterogeneity, and complex combination of physicochemical and biological properties. To address this evolving topic, the International Society for Extracellular Vesicles (ISEV) organized a dedicated workshop in Brescia, Italy, on 27th to 28th February, 2025. The event gathered selected participants from academia and industry, with expertise spanning synthetic nanomaterials, EVs, biophysics, and translational nanomedicine. The program included keynote lectures, oral presentations, posters, and a series of interactive roundtables structured around fundamentals of the biomolecular corona, EV corona biogenesis and composition, analytical and separation tools and clinical and diagnostic potential applications. This report summarizes the key scientific insights, methodological challenges, conceptual debates, and future perspectives that emerged from the workshop.