DOI: 10.4103/ijaim.ijaim_29_24 ISSN: 2772-6010
Bimodal Approach of Phytochemical Profiling of Panchakol Churna: A Polyherbal Formulation
Khushal Kumar, Sumedh Joshi, Shalini Rai, Anand B. More ABSTRACT
Introduction:
Phytochemicals from medicinal plants are crucial in traditional systems like Ayurveda and are increasingly important in modern healthcare. Panchakol Churna, a polyherbal Ayurvedic formulation, is known for various therapeutic properties. This study aims to profile the bioactive compounds in Panchakol Churna using a bimodal approach that combines the Indian Medicinal Plants, Phytochemistry and Therapeutics (IMPPAT) database with high-resolution liquid chromatography–mass spectrometry (HRLCMS).
Materials and Methods:
Panchakol Churna was prepared according to the Ayurvedic Formulary of India, with ingredients authenticated by the Regional Raw Drug Repository at AIIA, New Delhi. Phytochemical identification involved the IMPPAT 2.0 database and HRLCMS analysis using an Agilent G6550A MS Q-TOF system in both positive and negative ionization modes. Data analysis with Venny 2.1 identified common phytoconstituents across both ionization modes.
Results and Discussion:
HRLCMS identified 200 compounds, 100 in each mode, with 45 and 81 major compounds confirmed. Venny 2.1 revealed 127 common compounds between the two modes, highlighting the common constituents of Panchakol Churna. No overlap was observed between the phytoconstituents identified by IMPPAT and HRLCMS.
Conclusion:
The lack of overlap between the IMPPAT and HRLCMS results underscores the formulation’s complexity and suggests further exploration of its therapeutic potential. The findings support the development of a chemical library for medicinal plants, facilitating future pharmacological research and the authentication of medicinal plants.