Bacterial sialidases subvert the complement system through desialylation

Abstract

Bacterial sialidases (neuraminidases) are widely studied virulence factors, yet their impact on the complement system, a central component of innate immunity, remains poorly understood. Here, we investigate the effects of six sialidases from five human pathogens, including Bacteroides fragilis , Clostridium perfringens , Tannerella forsythia , Treponema denticola , and Vibrio cholerae on complement immunity using an integrated approach combining genetics, biochemistry, glycobiology, mass spectrometry, and structural biology. We show that major complement proteins, including C1q, C4, and C5, as well as complement regulators Factor I, Factor H, and C4BP, are modified by terminal sialic acids (sialylation) and undergo terminal desialylation by all six sialidases. Using the oral pathogen T. denticola and its sialidase NanH as a representative model, we demonstrate that sialidase‐mediated desialylation suppresses complement activation and surface deposition, promoting complement evasion. Structural analyses of T. denticola NanH in complex with an inhibitor, sialic acid, and native glycans, together with comparisons to other sialidase structures, reveal the molecular basis of substrate recognition and catalysis. Despite substantial sequence diversity, all six exo‐ α ‐sialidases share a conserved six‐bladed β ‐propeller catalytic domain and cleave both N‐acetylneuraminic acid and N‐glycolylneuraminic acid, the predominant mammalian sialic acids. Together, these findings establish a conserved structural and functional mechanism by which diverse bacterial pathogens desialylate complement components to impair innate immune defense.