Association of Th2-like inflammation with Tfh/Tph–germinal center immune programmes in submandibular gland lesions of IgG4-related disease
Motohisa Yamamoto, Ryuta Kamekura, Masaaki Uehara, Yuta Ichii, Kenichi TakanoAbstract
Objectives
IgG4-related disease (IgG4-RD) has long been associated with Th2-predominant immune responses and allergic features. However, the immunological context underlying lesional Th2-like inflammation remains incompletely understood.
Methods
Bulk RNA-seq data from 49 submandibular gland lesions of IgG4-RD were analyzed. Module scores representing Th2, Tfh/Tph, B cell, germinal center B cell (GC_B), plasma cell, macrophage and stromal/fibrotic programmes were calculated. Correlations between Th2 module scores and other immune/stromal modules were assessed using Spearman correlation analysis. Differentially expressed genes between Th2-high and Th2-low lesions were also evaluated.
Results
Th2 module scores were strongly associated with Tfh, Tfh/Tph and Tph helper T cell programmes, as well as with B cell, GC_B and plasma cell signatures. In addition, Th2 module scores positively correlated with M2 macrophage and fibrosis-associated macrophage (FAM) programmes. Differential expression analysis demonstrated increased expression of chronic helper T cell-related genes, including PDCD1, TOX and MAF, together with plasmablast-associated genes such as JCHAIN, MZB1 and PRDM1 in Th2-high lesions. In contrast, serum IgG4 and IgE levels did not significantly differ between Th2-high and Th2-low groups.
Conclusions
Lesional Th2-like inflammation in IgG4-RD was closely associated with chronic Tfh/Tph–germinal center immune programmes linked to plasmablast and M2/FAM macrophage responses. No clear association with available systemic allergy-related parameters was observed in this cohort.