Assessment of microvessel density in breast carcinoma using CD34 and CD105 immunohistochemistry
Choudhary Ravi Ray, Anju Singh, Guddi Rani Singh, Rashmi Rani Bharti, Manish KumarAbstract
Background:
High microvessel density (MVD) levels are associated with tumor aggressiveness and poor survival outcomes. The quantification of angiogenesis through MVD assessment using CD34 and CD105 has shown prognostic value in breast carcinoma.
Aims:
The study focuses on evaluating the role of CD34 and CD105 in determining MVD in breast carcinoma and their correlation with tumor grade and prognosis.
Materials and Methods:
The study included 50 invasive breast carcinomas. Clinicopathological parameters were recorded, and assessment of MVD was done immunohistochemically using CD34 and CD105. Association of clinicopathological findings with MVD (separately for CD34 and CD105) was determined.
Results:
Younger patients (≤40 years), premenopausal women, and those with high socioeconomic status exhibited significantly higher MVD for CD34 and CD105, indicating enhanced angiogenesis. Upper inner quadrant tumors had the highest MVD with a significant association. Smaller tumors (≤2 cm, pT1), unifocality, grade 3 and Stage III cancers, invasive ductal carcinoma not otherwise specified, and lymph node positivity had significantly higher MVD.
Conclusion:
Increased angiogenesis is significantly associated with younger age, premenopausal status, higher socioeconomic status, smaller tumor size, unifocality, higher tumor grade and stage, and lymph node positivity. Notably, node positivity and high socioeconomic status emerged as independent predictors of elevated MVD in multivariate analysis. These findings suggest that tumor angiogenesis is linked to tumor aggressiveness, and variation of MVD with demographic and pathological parameters reinforces the potential of CD105 and CD34 as valuable prognostic markers.