DOI: 10.2144/btn-2023-0104 ISSN: 0736-6205

Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay

Seongsoo Hong, Jeongho Park, Jaekyung Bok, Euna Cho, Joowon Rhee
  • General Biochemistry, Genetics and Molecular Biology
  • Biotechnology

A colorimetric loop-mediated isothermal amplification assay detects changes in pH during amplification based on color changes at a constant temperature. Currently, various studies have focused on developing and assessing molecular point-of-care testing instruments. In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96–0.99) and 0.96 (95% CI: 0.92–0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.

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