Application of Thin Layer Chromatography in Determining the Natural Diversifications of Phytochemicals in Rubia cordifolia
Kalyan Hazra, Deepak Kumar, Ajay Kumar Meena, Preeti Sharma, Gajji BabuIntroduction:
Geographical location plays a crucial role in the distribution and potency of medicinal plants. Chemical composition of plants and their medicinal properties can be signifi-cantly influenced by climate and soil composition. The present objective is to investigate the manifestation in phytocompounds of Rubia cordifolia L. root originating from different biogeo-graphical locations in the Indian subcontinent. R. cordifolia is traditionally used to treat chronic pyrexia and puerperal fever, along with reducing the heat and itching in various skin diseases. Other therapeutic benefits include immune-modulatory, analgesic, diuretic, hepatoprotective, nephroprotective, gastroprotective, wound-healing, and antiviral properties.
Methods:
This study establishes a qualitative profile and analyzes the amount of rubiadin in the root of Rubia cordifolia L. across four different biogeographical zones by using a reproducible and validated HPTLC method.
Results:
The study revealed phytochemical arrays are not identical for plants of different zones and that the quantity of rubiadin was highest (0.73 %) and lowest (0.27%) in the plants grown in the Deccan Plateau region and northeast region, respectively.
Discussion:
The study demonstrates that the Indian subcontinent boasts a rich tapestry of biodi-versity, and this diversity is reflected in the array of phytocompounds found across its various bi-ogeographical regions, mainly due to climate and soil characteristics.
Conclusion:
This information can be valuable for applications in agriculture, pharmaceuticals, and environmental studies because understanding the distribution and concentration of phyto-compounds across different locations can have numerous applications in these fields.