Application of scot markers for evaluation of the genetic variability of blue-grass varieties (Poa pratensis L.)

The analysis was conducted to assess the inter-varietal genetic polymorphism of Poa pratensis based on PCR technology using nine SCoT markers. The informative primers for variety differentiation have been identified; parameters of genetic diversity were estimated: Shannon’s index, Nei’s genetic diversity index, effective number of alleles and the indicator of the information content of primers (PIC — polymorphism information content). Genetic structure of the analyzed collection was evaluated using the Neighbor-Joining dendrogram and PCoA-analysis. As a result of the research, molecular formulas were developed that can be used as the individual characteristic of the variety originality and for DNA certification.