Dewa Ayu Agung Diah Hadiningrat, Agung Wiwiek Indrayani, Ketut Kwartantaya Winaya

Application of Arabica Coffee (Coffea arabica) Leaf Ethanol Extract Gel Reduces Expression of Matrix Metalloproteinase-1 and Increases the Amount of Collagen in Male Wistar Rats Exposed to Ultraviolet B Light

  • General Mathematics

Introduction: This study aims to determine the effect of arabica coffee leaf ethanol extract gel on decreasing MMP-1 expression and increasing the amount of collagen in the skin of male Wistar rats (Strains) exposed to UV-B light. Methods: This is a post-test only control group design experimental study using male rats as subjects. Each group was exposed to UV light and smeared with 5% coffee leaf ethanol extract cream every day. Then the back skin tissue was taken and the amount of collagen was examined by Picro Sirius Red staining and MMP1 levels were examined by ELISA staining. Results: The mean MMP-1 levels in groups N, K, P1, P2, and P3 were 1.023±0.018 ng/ml, 1.578±0.28 ng/ml, 1.001±0.027 ng/ml, 0.775±0.042 ng/ml, and 0.595±0.076 ng/ml. The average percentage of collagen in groups N, K, P1, P2, and P3 was 75.63 ± 2.92%, 66.43 ± 1.67%, 72.83 ± 3.13%, 76.02 ± 3 .78, and 80.43 ± 2.12%. MMP-1 levels decreased with increasing doses of arabica coffee leaf extract gel. The mean percentage of collagen increased with increasing doses of arabica coffee leaf extract gel in experimental animals. Conclusion: Arabica coffee leaf ethanol extract gel inhibited the increase in MMP-1 levels in male rats exposed to UV-B light. Administration of arabica coffee leaf ethanol extract gel can increase the amount of collagen in male rats exposed to UV-B light. Keywords: Arabica coffee, metalloprtoeinase-1, collagen, UVB.

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