DOI: 10.1177/1934578x261460594 ISSN: 1934-578X

Antioxidant Activity and Anti-glycation Effects of Nekemias grossedentata Leaf Ethanol Extract

Junhong Liu, Zengshang Wang, Ting Ruan, Qianghua Quan, Xue Shao, Guoqing Li
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Objectives

Nekemias grossedentata is a traditional herb known for its high flavonoid content, particularly dihydromyricetin (DMY), which exhibits a significant antioxidant and favorable safety profile, making it a promising candidate for mitigating skin aging. This study aims to develop a simplified and scalable protocol for obtaining N. grossedentata extract (NGE) with high-purity DMY, and to evaluate the antioxidant and anti-glycation activities of the extract.

Methods

A series of assays were conducted to evaluate the antioxidant activity of NGE, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Additionally, intracellular reactive oxygen species (ROS) level and the activities of antioxidant enzymes (superoxide dismutase, SOD; glutathione peroxidase, GSH-Px) were analyzed in H 2 O 2 -induced HaCaT cells. Anti-glycation activity was assessed by monitoring advanced glycation end-product (AGE) formation in Human primary dermal fibroblasts (HDFs)-methylglyoxal (MGO) systems.

Results

A rapid extraction method yielded NGE with 90.4% DMY purity. NGE exhibited potent radical scavenging activity (DPPH IC 50 = 6.89 ± 0.51 μg/mL; ABTS IC 50 = 4.37 ± 0.84 μg/mL). In cellular models, NGE significantly reduced H 2 O 2 -induced ROS and restored SOD and GSH-Px activities. Furthermore, NGE showed significant antiglycation activity (55.04%).

Conclusion

Through the synergistic integration of direct antioxidant interventions, enhancement of antioxidant enzyme activity, and anti-glycation mechanisms, NGE exhibits multi-target anti-aging properties. These findings underscore its potential as a natural ingredient in cosmetic and dermatological formulations.

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