Antioxidant activity according to bioactive compounds content in dried pumpkin waste
Sladjana Stajcic, Lato Pezo, Gordana Cetkovic, Jasna Canadanovic-Brunet, Anamarija Mandic, Vesna Tumbas-Saponjac, Jelena Vulic, Vanja Travicic, Miona Belovic- General Chemistry
In this study, for the preparation of dried pumpkin waste, freeze-drying and oven-drying (at 50?C and 65?C) were applied. Effect of drying methods on physical properties (moisture content, water activity, hygroscopicity, water solubility, bulk and tapped density, flowability and colour), content of bioactive compounds (carotenoids and phenolics) and antioxidant activity was investigated. Also, influence of one-year storage at -20?C on the bioactive compounds content and antioxidant activity of dried pumpkin waste was examined. Results indicated that drying method showed a significant impact on the investigated characteristics. Bioactive compounds content and antioxidant activity by DPPH test of freeze-dried were higher than of oven-dried pumpkin waste, while higher antioxidant activity in reducing power assay was determined by oven-dried pumpkin waste. An artificial neural network model was developed, for the anticipation of antioxidant activity according to bioactive compounds content (phenolics and carotenoids), in oven-dried (at 50?C and 65?C) and in freeze-dried pumpkin waste after one-year storage. These models showed good prediction properties (the r2 value during training cycle for output variables was 0.999). It was demonstrated that pumpkin waste is potentially an important source of bioactive compounds, which can be used after extraction in suitable forms in the development of functional food products.