DOI: 10.4103/bhsj.bhsj_10_26 ISSN: 2620-8636
Antileukemic Activity of Clitoria ternatea Extract and its Apoptotic Mechanism on Kasumi-1 Acute Myeloid Leukemia Cells
Nafhah, Enggar Yusrina Hasyyati, Arifa Mustika, Melissa Kilus, Nozlena Abdul Samad, Abdul Khairul Rizki Purba
Introduction:
Clitoria ternatea
(commonly known as bunga telang) is considered a medicinal herb. It contains high levels of polyphenols, including anthocyanins and triterpenoids, and has demonstrated anticancer properties against various types of cancer cells. Nevertheless, the effects of this plant on leukemia cells have not been extensively studied. This study aimed to establish if
C. ternatea
flower extract has any anticancer potential on Kasumi-1 cells, a renowned human acute myeloid leukemia (AML) cell line, and to analyze the half-mic (Half-maximal inhibitory concentration) variations over 24, 48, and 72 h.
Methods:
The study was done
in vitro
using the Kasumi-1 AML cell line. Kasumi-1 cells were treated with various concentrations of
C. ternatea
extract (15.625–500 µg/mL). Cytotoxicity was assessed at 24, 48, and 72 h using the MTT assay. Differences between treatment groups to control were analysed using Student’s
t
-test
,
and
P
< 0.05 was regarded as statistically significant.
Results:
The extract induced dose-dependent cytotoxicity, as determined by the MTT assay. The greatest cytotoxic effect was observed at the 24-h time point (137.5 µg/mL), followed by reduced effects at 48 h (168 µg/mL) and 72 h (221 µg/mL).
Conclusions:
The extract of
C. ternatea
exhibited moderate cytotoxicity toward Kasumi-1 cells, with its potency decreasing over time. This reduction may be attributed to cellular adaptive mechanisms, although this was not investigated in the present study. Further studies are needed to elucidate the underlying mechanisms and evaluate the feasibility of
C. ternatea
as a potential therapeutic agent for leukemia treatment.