DOI: 10.1111/irv.70166 ISSN: 1750-2640

Anticoronavirus Isoquinoline Alkaloids: Unraveling the Secrets of Their Structure–Activity Relationship

Marcela Safratova, Yu‐Li Chen, Anna Hostalkova, Jakub Chlebek, Chung‐Fan Hsieh, Bing‐Hung Chen, Lucie Cahlikova, Stefan Kosturko, Anders Backlund, Jim‐Tong Horng, Tsong‐Long Hwang, Michal Korinek
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ABSTRACT

Background

Natural alkaloids are a structurally diverse class of bioactive compounds with significant therapeutic potential. This study aimed to evaluate the in vitro antiviral activity of various natural alkaloids against coronaviruses, clarify molecular effects via bioassays and docking, and explore structure–activity relationships. Tested compounds included a wide variety of isoquinoline and Amaryllidaceae‐type alkaloids.

Methodology

Antiviral activity was assessed using HCoV‐229E and pseudotyped lentivirus assays for different strains of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Cytotoxicity was evaluated with the WST‐1 assay. AutoDock was used for molecular docking, online tools assessed drug‐likeness, and ChemGPS‐NP analyzed physicochemical properties correlated to antiviral clinical drugs.

Results

Several bis‐benzylisoquinoline alkaloids, especially from Berberis vulgaris L., and specific Amaryllidaceae alkaloids showed protective activity against HCoV‐229E (EC50 = 4.1–8.1 μM). Active compounds were further tested against SARS‐CoV‐2 variants. Aromoline (Compound 16) exhibited strong antiviral activity, inhibiting D614G, Delta, and Omicron variants in pseudovirus assays with IC50 values of 0.47–0.66 μM. Other bis‐benzylisoquinoline analogues showed moderate activity (IC50 = 1.24–2.86 μM). Docking studies revealed aromoline's favorable interaction at the SARS‐CoV‐2 spike/ACE2 interface, forming hydrogen bonds with Gln493 and Ser494 (binding energy −5.34 kcal/mol). ChemGPS‐NP analysis highlighted a distinct cluster of active bis‐benzylisoquinolines (Compounds 16–19) in chemical space.

Conclusion

This study highlights the antiviral potential of bis‐benzylisoquinoline and Amaryllidaceae alkaloids, particularly aromoline. The findings support their relevance as scaffolds for developing novel anticoronavirus agents and advance the understanding of their structure–activity relationships.

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