Angucyclinones rescue PhLOPS _A antibiotic activity by inhibiting Cfr-dependent antibiotic resistance

ABSTRACT

Methylation of the ribosome is a prevalent strategy conferring resistance to antibiotics that inhibit protein synthesis. Methylation is catalyzed by several distinct enzymes, many encoded on mobile genetic elements widespread in bacteria. One enzyme that is increasingly common in Gram-positive pathogens is Cfr. This radical S-adenosyl-L-methionine rRNA methylase confers resistance to the antibiotics

enicols, incosamides, xazolidinones, leuromutilins, and treptogramin (PhLOPS _A via methylation of A2503 of the 23S rRNA of the large ribosomal subunit. One strategy to safeguard the utility of these antibiotics is to identify inhibitors of Cfr-dependent methylation that may rescue antibiotic activity. We screened our in-house library of microbial natural product extracts against Cfr ⁺ Escherichia coli and identified an extract from Streptomyces WAC01849 that potentiated the PhLOPS _A antibiotic linezolid in a Cfr-dependent manner. We isolated the active angucyclinones 8- O -methyltetrangomycin (MTN) and 8- O -methyltetrangulol (MTL) from this extract. Both compounds selectively potentiated representatives of the PhLOPS _A antibiotics in Escherichia coli and methicillin-resistant Staphylococcus aureus in a Cfr-dependent manner. Finally, we observed inhibition of Cfr-mediated ribosome methylation in cells treated with MTN and MTL. Our findings suggest that MTN and MTL interfere with Cfr-dependent methylation to protect the efficacy of the PhLOPS _A antibiotics and offer a unique chemical scaffold that may be applied in developing clinically useful Cfr inhibitors.

IMPORTANCE

Cfr is an antibiotic resistance enzyme that inhibits five clinically important antibiotic classes, is genetically mobile, and has a minimal fitness cost, making Cfr a serious threat to antibiotic efficacy. The significance of our work is in discovering molecules that inhibit Cfr-dependent methylation of the ribosome, thus protecting the efficacy of the PhLOPS _A antibiotics. These molecules are the first reported inhibitors of Cfr-mediated ribosome methylation and, as such, will guide the further discovery of chemical scaffolds against Cfr-mediated antibiotic resistance. Our work acts as a foundation for further development of molecules that safeguard the PhLOPS _A antibiotics from Cfr.