W. G. Starkey, D. C. A. Candy, D. Thornber, J. Collins, A. J. Spencer, M. P. Osborne, J. Stephen

An in Vitro Model to Study Aspects of the Pathophysiology of Murine Rotavirus‐Induced Diarrhoea

  • Gastroenterology
  • Pediatrics, Perinatology and Child Health

SummaryAn in vitro system is described and validated for studying transport of solutes and water in both uninfected and rotavirus‐infected neonatal mouse intestine. Control intestine exhibited stable water absorption for periods of up to 40 min. Water absorption was temperature‐dependent, Na‐dependent, and enhanced by glucose‐containing perfusion solutions. Theophylline induced net secretion of water by control intestinal tissue. Water transport by rotavirus‐infected lower small intestine was significantly depressed as compared to control levels, and rotavirus‐infected middle small intestine exhibited net secretion of water. Upper small intestine and colon from infected animals did not differ significantly from control tissues in their ability to transport water. Water secretion by infected middle small intestine was reversed to absorption by glucose‐containing solutions.

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

  • Web-based, modern reference management
  • Collaborate and share with fellow researchers
  • Integration with Overleaf
  • Comprehensive BibTeX/BibLaTeX support
  • Save articles and websites directly from your browser
  • Search for new articles from a database of tens of millions of references
Try out CiteDrive

More from our Archive