Agromyces capsici sp. nov., Agromyces cucurbitae sp. nov., and Agromyces solani sp. nov.: Three New Species Isolated from Rhizosphere Soils in Yunnan Province, China

Three Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strains, designated LJC-15T, NGA-4T and QZ2-6-4T, were isolated from the rhizosphere soils of Capsicum, Cucurbita and Solanum melongena, respectively, in Yunnan Province, China. Phylogenetic and phylogenomic analyses placed the three strains within the genus Agromyces but distinguished them from recognized species. All ANI and dDDH values between the novel strains and phylogenetically related type strains were well below the generally accepted species delineation thresholds. The three strains could also be differentiated from selected reference type strains by phenotypic characteristics. Chemotaxonomically, they contained MK-12 as the predominant menaquinone and anteiso-C15:0, anteiso-C17:0 and iso-C16:0 as the major fatty acids, while their whole-cell sugar and polar lipid profiles were consistent with those characteristic of the genus Agromyces. On the basis of the combined phylogenetic, genomic, phenotypic and chemotaxonomic evidence, strains LJC-15T, NGA-4T and QZ2-6-4T are proposed to represent three novel species of the genus Agromyces, for which the names Agromyces capsici sp. nov., Agromyces cucurbitae sp. nov. and Agromyces solani sp. nov. are proposed, respectively. The type strains are LJC-15T (=GDMCC 1.6754T = KCTC 59690T), NGA-4T (=GDMCC 1.6755T = KCTC 59691T) and QZ2-6-4T (=GDMCC 1.6756T = KCTC 59692T), respectively.