Advancements in Chiral Separation and Emerging Alternative Techniques: Recent Trends and Future Perspectives
Saloni Kumari, Pawan Kumar Goswami, Arvind Kumar Patel, Sandip Chatterjee, Siddhant KrishnaIntroduction:
Despite its importance in a wide range of applications, chirality has a special position in pharmaceutical, chemical, and biological sciences because biological systems act in an enantioselective manner. Owing to the large differences in their pharmacokinetics, pharmacodynamics, and toxicity, enantiomers of chiral compounds often possess extremely different profiles, making accurate chiral separation an essential part of drug discovery, quality control, and regulatory forensics. Due to rapid progress in analytical science, a variety of cutting-edge chiral separation methods are now available beyond classical statistics.
Methods:
This review highlights recent advances in chiral separation methods, including chromatographic, electrophoretic, and other novel approaches. Methods: We performed a literature search using major scientific databases, rigorous study selection, and qualitative synthesis of relevant findings.
Results:
This study covers the development of new chiral stationary phases; thus, many important developments have been made in the area of chiral separation, including immobilized polysaccharides, nanoparticles, and metal-organic frameworks, to increase the performance of chiral stationary phase design. Progress in advanced methods, such as supercritical fluid chromatography, twodimensional chromatography, and capillary electrophoresis, illustrates higher efficiency, lower solvent usage, and better sustainability. We further factor in analytical performance via seamless integration with hyphenated detection systems and artificial intelligence–assisted method development.
Discussion:
Although modern chiral separation strategies continue to embrace the principles of green analytical chemistry, they are also being driven towards expansion by a push for automation and predictive modeling. Nevertheless, the high cost, absence of standalone universal selectors, and inadequate extrapolation of the methods remain challenges, but strides in innovation indicate an oncoming trend towards more intelligent, scalable, and rapid analytical platforms
Conclusion:
The burgeoning development of chiral separation technologies catapults enantioselective analysis into new territories, paving the way for safer pharmaceuticals, regulatory compliance, and, ultimately, precision medicine. Future trends and progress in this field will be characterized by the further integration of material science, digital tools, and sustainable practices.