Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen
Suherni Susilowati, Imam Mustofa, Tri Wahyu Suparyogi, Adeyinka Oye Akintunde, Djoko Agus Purwanto, Wurlina Wurlina, Suzanita Utama, Sri Mulyati- Obstetrics and Gynecology
- Plant Science
- General Veterinary
- Animal Science and Zoology
- Reproductive Medicine
Objective:
To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent, and the proper thawing temperature.
Methods:
The ejaculate of Boer buck was added to skimmed egg yolk diluent without (the control group) and with adding 1 μg of chitosan nanoparticles of green tea extract per mL of diluent (the treatment group). Then, the diluted semen was filled in French mini straws containing 60× 106 live sperm per straw, frozen in a standard protocol, and stored as frozen semen at -196 °C for a week. Six replicates from each group were diluted for 30 s at 37 °C or 39 °C sterile water to evaluate the semen quality.
Results:
Post-thawing (at 37 °C or 39 °C) of live sperm, progressive motility, and plasma membrane integrity were lower compared to those of the pre-freezing stage (
Conclusions:
Higher quality post-thawing Boer buck semen is achieved by adding 1 μg/mL of chitosan nanoparticles of green tea extract to the skimmed egg yolk diluent and thawing at 39 °C.